Interspersed repetitive sequence (IRS)-PCR for typing of whole genome radiation hybrid panels
نویسندگان
چکیده
منابع مشابه
YAC subclone contig assembly by serial interspersed repetitive sequence (IRS)-PCR product hybridizations.
Yeast artificial chromosomes (YACs) are presently the most effective means of cloning large contiguous regions of genomic DNA (1). However, performing a detailed molecular analysis of YAC clone insert DNA is constrained by inherent physical and technical limitations. Typically, an extended analysis of YAC DNA is facilitated by subcloning YAC insert DNA into lambda bacteriophage or cosmid vector...
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Using two recently developed techniques, IRS-PCR YAC walking and IRS-PCR genotyping, a framework-integrated physical and genetic map of the mouse genome was constructed. The map consists of 821 contigs, containing 7746 YAC clones originating from three different YAC libraries. Three hundred eighty of the contigs have been anchored to the genetic map. Approximately 16% of the physical length of ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2000
ISSN: 1362-4962
DOI: 10.1093/nar/28.2.e7